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When studies are performed in human primary models or in established and transformed cell lines in conditions requiring expensive reagents, analysis has to be performed with a sensitive but reliable technique because only a limited number of experiments can be run on each sample. We have often faced the problem of detecting poorly expressed transcripts, as well as that of handling small amounts of unique samples, such as primary hematopoietic cells ( 3, 4) or tumor biopsies from human patients ( 5- 7). A common question is the quantification of specific RNA transcripts and the detection of any variation in their expression levels under different experimental conditions. In most cases, when RNA analysis is required, a qualitative study is not sufficient to deliver a satisfactory answer. 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